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non fat dried milk  (Valiant Co Ltd)

 
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    Valiant Co Ltd non fat dried milk
    Non Fat Dried Milk, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non fat dried milk/product/Valiant Co Ltd
    Average 94 stars, based on 43 article reviews
    non fat dried milk - by Bioz Stars, 2026-06
    94/100 stars

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    Representative ITC thermograms (top panel) and corresponding integrated binding isotherms (bottom panel) for (A) WT FGF1 and (B) FLEX titrated with polymeric heparin from three independent experiments. Experiments were performed at 25 °C in Tris pH 7.4 with 100 mM NaCl. Data were fitted using a single-site binding model.

    Journal: bioRxiv

    Article Title: FLEX: A heparin-binding fusion partner engineered from fibroblast growth factor 1 to enhance protein expression, solubility and purity

    doi: 10.64898/2026.03.17.712271

    Figure Lengend Snippet: Representative ITC thermograms (top panel) and corresponding integrated binding isotherms (bottom panel) for (A) WT FGF1 and (B) FLEX titrated with polymeric heparin from three independent experiments. Experiments were performed at 25 °C in Tris pH 7.4 with 100 mM NaCl. Data were fitted using a single-site binding model.

    Article Snippet: Membranes were blocked in Tris-buffered saline with 0.1% (v/v) Tween 20 (TBS-T) in 5% (w/v) non-fat dried milk (pH 7.4) followed by incubation with primary anti-6His tag antibody (Bio-Rad or Cell Signalling Technologies) and secondary horse radish peroxidase-conjugated anti-mouse (ThermoFisher) antibody.

    Techniques: Binding Assay